Optimasi sistem kultur dan media untuk peningkatan tinggi tunas in vitro kelapa sawit

Optimization of in vitro shoot growth is necessary to shorten the culture time and produce vigorous oil palm plantlets. This research was conducted determine best media techniques accelerate palm. Shoots derived from somatic embryogenesis were grown on DF with two systems (solid medium double-layer) combined hormone treatments (0.5-1 mg L-1 giberellin/GA3 0.5 Benzyl amino purine/BAP or thidiazuron/ TDZ). Further optimization using different bottle closures (polypropylene screw caps plastic wraps) macronutrients (standard double concentrations). a completely randomized design (CRD), each treatment consisting 5 replications shoots. The results showed that double-layer system GA3 L-1) TDZ (0.5 gave highest height increment. use double-strength macronutrient increased (136-167 %) decreased tip necrosis (0-24 %). Plastic wrap (STN), while doubling reduced STN. shoots began slow down after weeks culture. In conclusion, optimal conditions for at usage added 0.5-1 L-1, jars polypropylene caps.